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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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factor kappa b ligand  (Santa Cruz Biotechnology)
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). <t>K15+</t> ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).
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Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). K15+ ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: 1α,25-Dihydroxyvitamin D3 accelerates skin wound re-epithelialization by promoting epidermal stem cell proliferation and differentiation through PI3K activation: an in vitro and in vivo study

doi: 10.1590/1414-431X2025e14121

Figure Lengend Snippet: Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in wounds. A , Representative immunohistochemical-stained sections of PCNA expression. Scale bars: 200 μm (low magnification); 100 μm (high magnification). K15+ ( B ) and CD49f+/CD71- ( C ) cells from wound tissues on the 5th day after wounding treated with different doses of vitamin D (0, 5, 50, 250 ng) were detected by flow cytometry (n=3/group). Data are reported as means±SD. **P<0.01 compared with control group (ANOVA).

Article Snippet: The antibodies used were CD49f-BV421 (1:200, BioLegend, 313624, USA), CD71-PE (1:200, Invitrogen, 2024832, USA), K15-PE (1:200, Santa Cruz Biotechnology, sc-47679, USA), K14-AF488 (1:200, Santa Cruz Biotechnology, sc-53253), and K10-PE (1:200, Santa Cruz Biotechnology, sc-53251).

Techniques: Immunohistochemical staining, Staining, Expressing, Flow Cytometry, Control

Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in vitro . A , The proliferation of skin epidermal stem cells from C57BL/6 mice treated with different doses of vitamin D3 (0, 1, 10, 50 nM) for 48 h was detected by CCK8 (n=3/group). K15+ ( B ), CD49f +/CD71- ( C ), K14+ ( D ), and K10+ ( E ) cells were detected by flow cytometry after the EpSCs were treated with different doses of vitamin D (0, 1, 10, 50 nM) for 48 h, and quantitative data (right panel) (n=3/group). Data are reported as means±SD. *P<0.05 and **P<0.01 compared with control group (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: 1α,25-Dihydroxyvitamin D3 accelerates skin wound re-epithelialization by promoting epidermal stem cell proliferation and differentiation through PI3K activation: an in vitro and in vivo study

doi: 10.1590/1414-431X2025e14121

Figure Lengend Snippet: Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in vitro . A , The proliferation of skin epidermal stem cells from C57BL/6 mice treated with different doses of vitamin D3 (0, 1, 10, 50 nM) for 48 h was detected by CCK8 (n=3/group). K15+ ( B ), CD49f +/CD71- ( C ), K14+ ( D ), and K10+ ( E ) cells were detected by flow cytometry after the EpSCs were treated with different doses of vitamin D (0, 1, 10, 50 nM) for 48 h, and quantitative data (right panel) (n=3/group). Data are reported as means±SD. *P<0.05 and **P<0.01 compared with control group (ANOVA).

Article Snippet: The antibodies used were CD49f-BV421 (1:200, BioLegend, 313624, USA), CD71-PE (1:200, Invitrogen, 2024832, USA), K15-PE (1:200, Santa Cruz Biotechnology, sc-47679, USA), K14-AF488 (1:200, Santa Cruz Biotechnology, sc-53253), and K10-PE (1:200, Santa Cruz Biotechnology, sc-53251).

Techniques: In Vitro, Flow Cytometry, Control